Journal: Human Reproduction (Oxford, England)

Article Title: Chronic hyperandrogenemia in the presence and absence of a western-style diet impairs ovarian and uterine structure/function in young adult rhesus monkeys

doi: 10.1093/humrep/dex338

Figure Lengend Snippet: Photomicrographs illustrating immunohistochemical staining for estrogen receptor 1 (ESR1), progesterone receptor (PGR) MMP26, TIMP3, Ki-67 and androgen receptor (AR) in the endometrial functionalis zone of the macaque uterus from representative females in each treatment group (C, T, WSD, T+WSD). Brown staining denotes positive expression of proteins. Sections are counterstained with hematoxylin (blue) staining. ESR1, PGR, Ki-67 and AR staining is nuclear, while MMP26 and TIMP3 show cytoplasmic localization. Inset shows a negative control with an irrelevant antibody (Anti-Br(d)U). TIMP3 staining was localized to the predecidual cells around the spiral arteries.

Article Snippet: Antibodies used were against estrogen receptor 1 (ESR1,ER-ID5; Cat#: MS-354-P, Thermo Fisher Scientific), progesterone receptor (PGR, Cat#: Ms-298-P, 1 μg, Lab Vision/NeoMarkers, Fremont, CA, USA), androgen receptor (AR-F39 (Cat#: MU256, 1/50, BioGenex, Fremont, CA, USA), Ki67 (Cat#: MU370-UC, 1/200, BioGenex), MMP26 (Cat# ab57636; Abcam, Cambridge, MA, USA) and TIMP3 (Cat#:MAB973, R&D Systems, Inc. Minneapolis, MN, USA).

Techniques: Immunohistochemical staining, Staining, Expressing, Negative Control

Journal: Human Reproduction (Oxford, England)

Article Title: Chronic hyperandrogenemia in the presence and absence of a western-style diet impairs ovarian and uterine structure/function in young adult rhesus monkeys

doi: 10.1093/humrep/dex338

Figure Lengend Snippet: Expression of ESR1, PGR, MMP26 and TIMP3 mRNAs as detected by qRT-PCR in endometrial biopsies collected from macaques in the mid-luteal phase of the cycle. Significant (P < 0.05) differences between individual treatment groups are denoted by different uppercase letters above columns.

Article Snippet: Antibodies used were against estrogen receptor 1 (ESR1,ER-ID5; Cat#: MS-354-P, Thermo Fisher Scientific), progesterone receptor (PGR, Cat#: Ms-298-P, 1 μg, Lab Vision/NeoMarkers, Fremont, CA, USA), androgen receptor (AR-F39 (Cat#: MU256, 1/50, BioGenex, Fremont, CA, USA), Ki67 (Cat#: MU370-UC, 1/200, BioGenex), MMP26 (Cat# ab57636; Abcam, Cambridge, MA, USA) and TIMP3 (Cat#:MAB973, R&D Systems, Inc. Minneapolis, MN, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: Transplantation

Article Title: Urinary TIMP-2 Predicts the Presence and Duration of Delayed Graft Function in Donation After Circulatory Death Kidney Transplant Recipients

doi: 10.1097/tp.0000000000002472

Figure Lengend Snippet: FIGURE 2. TIMP-2 and IGFBP7 values were significantly different for patients with and without fDGF. Median ± IQR of both TIMP-2 and IGFBP7 are plotted for patients with and without fDGF. TIMP-2 values were significantly higher for patients with fDGF as compared to patients without fDGF (A). This also applied for the IGFBP7 values (B). Interestingly, TIMP-2 values of all patients decreased in the first 10 days after transplantation, where as IGFBP7 values increased. fDGF, functionally defined delayed graft function; IGFBP7, insulin-like growth factor-binding protein 7; IQR, interquartile range; TIMP-2, tissue inhibitor of metalloproteinases-2.

Article Snippet: Urinary TIMP-2 and IGFBP7 were quantified using sandwich immunoassays (ELISA, DTM200; R&D Systems, Minneapolis, MN for TIMP-2 and EK0991, Boster Biological Technology, Pleasanton, CA for IGFBP7, respectively) according to the manufacturer’s instructions.

Techniques: Transplantation Assay, Binding Assay

Journal: Transplantation

Article Title: Urinary TIMP-2 Predicts the Presence and Duration of Delayed Graft Function in Donation After Circulatory Death Kidney Transplant Recipients

doi: 10.1097/tp.0000000000002472

Figure Lengend Snippet: FIGURE 3. ROC curves of TIMP-2 and IGFBP7 in the prediction of fDGF and prolonged fDGF The absolute concentration of TIMP-2 on day 1 could adequately identify patients with fDGF, and this further improved in case TIMP-2 was corrected for osmolality (TIMP-2/ mOsm) (A). The concentration of IGFBP7 on day 1, on the other hand, showed only limited predictive value also when corrected for osmolality (IGFBP7/mOsm) (B). On day 10, both TIMP-2 and TIMP-2/mOsm could also adequately predict prolonged fDGF duration ≥21 days (C), whereas IGFBP7 and IGFBP7/mOsm could not (D). fDGF, functionally defined delayed graft function; IGFBP7, insulin-like growth factor-binding protein 7; ROC, receiver operating characteristic; TIMP-2, tissue inhibitor of metalloproteinases-2.

Article Snippet: Urinary TIMP-2 and IGFBP7 were quantified using sandwich immunoassays (ELISA, DTM200; R&D Systems, Minneapolis, MN for TIMP-2 and EK0991, Boster Biological Technology, Pleasanton, CA for IGFBP7, respectively) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Binding Assay

Journal: Transplantation

Article Title: Urinary TIMP-2 Predicts the Presence and Duration of Delayed Graft Function in Donation After Circulatory Death Kidney Transplant Recipients

doi: 10.1097/tp.0000000000002472

Figure Lengend Snippet: FIGURE 5. TIMP-2/mOsm and IGFBP7/mOsm in tertiles. Patients were divided into tertiles based on TIMP-2/mOsm and IGFBP7/ mOsm concentrations, and the percentage of patients with fDGF and prolonged fDGF in each tertile was calculated. On day 1, patients without fDGF predominantly grouped together in the tertile with the lowest values for TIMP-2/mOsm, (77%), whereas patients with fDGF (92%) were in the middle or highest tertile (A). For IGFBP7/mOsm, a comparable figure was seen, with slightly lower percentages (B). On day 10, patients with prolonged fDGF predominantly grouped together in the tertile with the highest TIMP-2/mOsm values (83.3%), and none had TIMP-2/mOsm values in the lowest tertile (C). In contrast, patients with prolonged fDGF did not group together according to their IGFBP7/mOsm values (D). fDGF, functionally defined delayed graft function; IGFBP7, insulin-like growth factor-binding protein 7; TIMP-2, tissue inhibitor of metalloproteinases-2.

Article Snippet: Urinary TIMP-2 and IGFBP7 were quantified using sandwich immunoassays (ELISA, DTM200; R&D Systems, Minneapolis, MN for TIMP-2 and EK0991, Boster Biological Technology, Pleasanton, CA for IGFBP7, respectively) according to the manufacturer’s instructions.

Techniques: Binding Assay